Composite

Part:BBa_K1520509:Design

Designed by: yue wu   Group: iGEM14_Nanjing-China   (2014-09-23)


PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3611
    Illegal NheI site found at 3634
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4812
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 548
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, tetR, Pcons2, lacI, Ter

References