Composite
Part:BBa_K1520509:Design
Designed by: yue wu Group: iGEM14_Nanjing-China (2014-09-23)
PgolTS-golS-PgolB-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-lacI-Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 3611
Illegal NheI site found at 3634 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4812
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 548
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
None
Source
PgolTS-golS-PgolB, tetR, PtetO, rfp, Plac, tetR, Pcons2, lacI, Ter